GPNMB Monoclonal antibody, PBS Only (Capture)

GPNMB Monoclonal Antibody for Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3E12

Cat no : 66926-2-PBS

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50045-1

  Sandwich ELISA standard curve of MP50045-1, GPNMB Monoclonal Matched Antibody Pair - PBS only. 66926-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag26747. 66926-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sandwich ELISA standard curve of MP50045-2

  Sandwich ELISA standard curve of MP50045-2, GPNMB Monoclonal Matched Antibody Pair - PBS only. 66926-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag26747. 66926-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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Product Information

66926-2-PBS targets GPNMB as part of a matched antibody pair:

MP50045-1: 66926-2-PBS capture and 66926-4-PBS detection (validated in Sandwich ELISA)

MP50045-2: 66926-2-PBS capture and 66926-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.03 x 10^-10M
  K_Off=1.53 x 10^-6M
  K_On=1.49 x 10^4M
• Immunogen: GPNMB fusion protein Ag26747
• Full Name: glycoprotein (transmembrane) nmb
• Calculated Molecular Weight: 64 kDa
• GenBank Accession Number: BC011595
• Gene Symbol: GPNMB
• Gene ID (NCBI): 10457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.