GPNMB Monoclonal antibody, PBS Only

GPNMB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B10B8

Cat no : 66926-1-PBS

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

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Validation Data Gallery

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  WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66926-1-Ig (same clone as 66926-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (66926-1-Ig, Clone: 2B10B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

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Product Information

66926-1-PBS targets GPNMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPNMB fusion protein Ag26747
• Full Name: glycoprotein (transmembrane) nmb
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC011595
• Gene Symbol: GPNMB
• Gene ID (NCBI): 10457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.