GPI Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GPI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11E4

Cat no : 67178-1-PBS

Synonyms

Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9, AMF

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Validation Data Gallery

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  WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

  WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

  WB result of GPI antibody (67178-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GPI antibody (67178-1-Ig, Clone: 2F11E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

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Product Information

67178-1-PBS targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPI fusion protein Ag7419
• Full Name: glucose phosphate isomerase
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC004982
• Gene Symbol: GPI
• Gene ID (NCBI): 2821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).