GPHN Monoclonal antibody, PBS Only

GPHN Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7C4

Cat no : 67995-1-PBS

Synonyms

Domain E, GEPH, gephyrin, GPH, GPHN, GPHRYN, KIAA1385, MPT adenylyltransferase, MPT Mo transferase

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Validation Data Gallery

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  WB analysis using 67995-1-Ig (same clone as 67995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67995-1-Ig (GPHN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67995-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67995-1-PBS targets GPHN in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPHN fusion protein Ag23708
• Full Name: gephyrin
• Calculated Molecular Weight: 769 aa, 83 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC030016
• Gene Symbol: GPHN
• Gene ID (NCBI): 10243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.