GPD2 Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68174-1-Ig (same clone as 68174-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50432-1

  Cytometric bead array standard curve of MP50432-1, GPD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68174-2-PBS. Detection antibody: 68174-1-PBS. Standard:Ag11212. Range: 0.391-200 ng/mL.

GPD2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 68174-1-PBS

Clone No.1C12E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

GPD M, GPD 2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GDH2, EC:1.1.5.3

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Product Information

68174-1-PBS targets GPD2 as part of a matched antibody pair:

MP50432-1: 68174-2-PBS capture and 68174-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPD2 fusion protein Ag11212
• Full Name: glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• Calculated Molecular Weight: 41 kDa, 81 kDa
• GenBank Accession Number: BC019874
• Gene Symbol: GPD2
• Gene ID (NCBI): 2820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P43304
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).