GPD1 Recombinant antibody, PBS Only (Capture)

GPD1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242359D3

Cat no : 84800-1-PBS

Synonyms

GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8, cGPDH

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Validation Data Gallery

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  WB analysis using 84800-1-RR (same clone as 84800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84800-1-RR (GPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84800-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84800-1-RR (same clone as 84800-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPD1 antibody (84800-1-RR, Clone: 242359D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84800-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01579-1

  Cytometric bead array standard curve of MP01579-1, GPD1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84800-1-PBS. Detection antibody: 84800-2-PBS. Standard: Ag4278. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 84800-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84800-1-RR against Human GPD1 were performed. The affinity constant is below 1 pM.

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Product Information

84800-1-PBS targets GPD1 as part of a matched antibody pair:

MP01579-1: 84800-1-PBS capture and 84800-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPD1 fusion protein Ag4278
• Full Name: glycerol-3-phosphate dehydrogenase 1 (soluble)
• Calculated Molecular Weight: 349 aa, 38 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC032234
• Gene Symbol: GPD1
• Gene ID (NCBI): 2819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.