GPAM Polyclonal antibody

GPAM Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12454-1-AP

Synonyms

GPAM, GPAT 1, GPAT1, KIAA1560, RP11 426E5.2

---

Validation Data Gallery

• 

  IP experiment of mouse liver using 12454-1-AP

  IP result of anti-GPAM (IP:12454-1-AP, 4ug; Detection:12454-1-AP 1:2000) with mouse liver tissue lysate 1840 ug.

• 

  IHC staining of rat brain using 12454-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12454-1-AP (GPAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12454-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12454-1-AP (GPAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12454-1-AP targets GPAM in IP, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPAM fusion protein Ag3120
• Full Name: glycerol-3-phosphate acyltransferase, mitochondrial
• Calculated Molecular Weight: 828 aa, 94 kDa
• Observed Molecular Weight: 90-92 kDa
• GenBank Accession Number: BC030783
• Gene Symbol: GPAM
• Gene ID (NCBI): 57678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.