Syntabulin Polyclonal antibody

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  WB analysis of HeLa using 16972-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16972-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 16972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 16972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16972-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16972-1-AP (Syntabulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Syntabulin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Cat No. 16972-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

Golgi-localized syntaphilin-related protein, GOLSYN, KIAA1472, SYBU, Syntaxin-1-binding protein

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

16972-1-AP targets Syntabulin in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9344

  Product name: Recombinant human GOLSYN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 128-485 aa of BC012082

  Sequence: SGRYMSCGENHGVRPPNPEQYLTPLQQKEVTVRHLKTKLKESERRLHERESEIVELKSQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSSLADKDKGIQKYFVDINIQNKKLESLLQSMEMAHSGSLRDELCLDFPCDSPEKSLTLNPPLDTMADGLSLEEQVTGEGADRELLVGDSIANSTDLFDEIVTATTTESGDLELVHSTPGANVLELLPIVMGQEEGSVVVERAVQTDVVPYSPAISELIQSVLQKLQDPCPSSLASPDESEPDSMESFPESLSALVVDLTPRNPNSAILLSPVETPYANVDAEVHANRLMRELDFAACVEERLDGVIPLARGGVVRQYWS

• Full Name: Golgi-localized protein
• Calculated Molecular Weight: 662 aa, 72 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC012082
• Gene Symbol: GOLSYN
• Gene ID (NCBI): 55638
• RRID: AB_1939519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX95
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Hum Mol Genet; Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.; Authors - Saravanan Gunaseelan
mouse,human	WB,IF	Cell Death Discov; Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B; Authors - Pingyang Ke
mouse	WB	Tissue Cell; Targeting Siglec-15 mediates mitochondrial retrograde regulation of cervical cancer development; Authors - Jing Wang
mouse	WB	Neurobiol Dis; Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.; Authors - Qi Wang