GOLGA5 Polyclonal antibody

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  WB analysis using 31164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31164-1-AP (GOLGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 31164-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA5 antibody (31164-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GOLGA5 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31164-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PIG31, GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells, mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31164-1-AP targets GOLGA5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GOLGA5 fusion protein Ag35031
• Full Name: golgi autoantigen, golgin subfamily a, 5
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83-95 kDa
• GenBank Accession Number: BC023021
• Gene Symbol: GOLGA5
• Gene ID (NCBI): 9950
• RRID: AB_3669878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TBA6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).