GNRHR Polyclonal antibody, PBS Only

GNRHR Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 19950-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 19950-1-AP (same clone as 19950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19950-1-AP (GNRHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19950-1-AP (GNRHR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19950-1-AP (GNRHR antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19950-1-AP (same clone as 19950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 19950-1-AP (same clone as 19950-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human GNRHR (19950-1-AP) (red) or 0.2 ug Control Antibody (blue), and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

---

Product Information

19950-1-PBS targets GNRHR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GnRH receptor
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: NM_000406
• Gene Symbol: GNRHR
• Gene ID (NCBI): 2798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.