GNPDA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GNPDA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12312-1-AP

Synonyms

GlcN6P deaminase 1, GNPDA, GNPDA 1, GNPDA1, GNPI, GPI, HLN, KIAA0060, Oscillin

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Validation Data Gallery

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  WB analysis of K-562 using 12312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12312-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12312-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 12312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12312-1-AP (GNPDA1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, human brain tissue, rat kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12312-1-AP targets GNPDA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNPDA1 fusion protein Ag2961
• Full Name: glucosamine-6-phosphate deaminase 1
• Calculated Molecular Weight: 289 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC012853
• Gene Symbol: GNPDA1
• Gene ID (NCBI): 10007
• RRID: AB_2110553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNPDA1(glucosamine-6-phosphate isomerase 1) is also named as GNPI, HLN, KIAA0060 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium(PMID:12965206). It may be playing an essential role for egg activation and early embryo development. GNPI is expressed as a 33-kD cytosolic protein in various cell lines(PMID: 9714720).

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	WB	Cancer Manag Res; Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma.; Authors - Dezhi Li ; KD Validated
human	WB	Glycobiology; Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.; Authors - Sanna Oikari ; KD Validated