GNPAT Monoclonal antibody, PBS Only

GNPAT Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5C10

Cat no : 66453-1-PBS

Synonyms

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

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Validation Data Gallery

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  WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 66453-1-Ig (same clone as 66453-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66453-1-Ig (same clone as 66453-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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Product Information

66453-1-PBS targets GNPAT in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNPAT fusion protein Ag6740
• Full Name: glyceronephosphate O-acyltransferase
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 65-69 kDa
• GenBank Accession Number: BC000450
• Gene Symbol: GNPAT
• Gene ID (NCBI): 8443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.