GNPAT Polyclonal antibody

• Featured Product
• KD/KO Validated

GNPAT Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14931-1-AP

Synonyms

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

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Validation Data Gallery

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  WB analysis of HepG2 using 14931-1-AP

  WB result of GNPAT antibody (14931-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNPAT transfected HepG2 cells.

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  WB analysis of HepG2 using 14931-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14931-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14931-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14931-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14931-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 14931-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14931-1-AP

  IP result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, human placenta tissue, Transfected HEK-293 cells, COLO 320 cells, HeLa cells, PC-3 cells, mouse brain tissue
• Positive IP detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

14931-1-AP targets GNPAT in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNPAT fusion protein Ag6735
• Full Name: glyceronephosphate O-acyltransferase
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 65-69 kDa
• GenBank Accession Number: BC000450
• Gene Symbol: GNPAT
• Gene ID (NCBI): 8443
• RRID: AB_2110546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID:21990100) This antibody recognize the 69 kDa GNPAT protein.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Functional multi-organelle units control inflammatory lipid metabolism of macrophages; Authors - Julia A Zimmermann
human	WB	Cell Host Microbe; Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.; Authors - Pierre M Jean Beltran ; KD Validated
mouse	WB	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park
human	IF	Int J Mol Sci; Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors.; Authors - Malin Tordis Meyer
human,hamster	WB	Hum Mutat; Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.; Authors - Itzkovitz Brandon B
mouse,rat	WB,IHC,IF	Front Cell Dev Biol; Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.; Authors - Rémi Karadayi ; KD Validated