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GNL3 Monoclonal antibody, PBS Only

GNL3 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B8F10

Cat no : 67169-1-PBS

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNL3 Monoclonal antibody (67169-1-Ig)

    WB analysis using 67169-1-Ig (same clone as 67169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67169-1-Ig (GNL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Monoclonal antibody (67169-1-Ig)

    IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

    Immunofluorescent analysis of 4% PFA fixed HeLa cells using 67169-1-Ig (GNL3 mouse antibody, green) at dilution of 1:200 and CoraLite488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Monoclonal antibody (67169-1-Ig)

    IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67169-1-Ig (GNL3 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67169-1-PBS targets GNL3 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GNL3 fusion protein Ag7056
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC001024
Gene Symbol GNL3
Gene ID (NCBI) 26354
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.