GNL2 Polyclonal antibody, PBS Only

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  WB analysis using 10190-2-AP (same clone as 10190-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10190-2-AP (GNL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10190-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10190-2-AP (same clone as 10190-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10190-2-AP (GNL2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10190-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10190-2-AP (same clone as 10190-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using GNL2 antibody (10190-2-AP) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10190-2-PBS in a different storage buffer formulation.

GNL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 10190-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

10190-2-PBS targets GNL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNL2 fusion protein Ag0243
• Full Name: guanine nucleotide binding protein-like 2 (nucleolar)
• Calculated Molecular Weight: 731 aa, 84 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000107
• Gene Symbol: GNL2
• Gene ID (NCBI): 29889
• RRID: AB_2110075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13823
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GNL2 ( G protein nucleolar 2) has a crucial role in the pathogenesis of gliomas by influencing key cellular processes, particularly enhanced protein synthesis, which ultimately contributes to the development of gliomas. In addition, GNL2 was found to affect the overall protein synthesis of ribosomal protein L11 in glioma cells. The calculated MW of GNL2 is 84 kDa, but the observed MW is around 100 kDa, which may be due to glycosylation modification.