GNE Monoclonal antibody, PBS Only

GNE Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11E10

Cat no : 67649-1-PBS

Synonyms

DMRV, GLCNE, GNE, IBM2, ManAc kinase, NM, Uae1, UDP GlcNAc 2 epimerase

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Validation Data Gallery

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  WB analysis using 67649-1-Ig (same clone as 67649-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67649-1-Ig (GNE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67649-1-PBS in a different storage buffer formulation.

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Product Information

67649-1-PBS targets GNE in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNE fusion protein Ag18702
• Full Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Calculated Molecular Weight: 722 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC121179
• Gene Symbol: GNE
• Gene ID (NCBI): 10020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.