GNB5 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GNB5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11045-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 11045-2-AP (same clone as 11045-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11045-2-AP (same clone as 11045-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 11045-2-AP (same clone as 11045-2-PBS)

  IP result of anti-GNB5 (IP:11045-2-AP, 4ug; Detection:11045-2-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11045-2-AP (same clone as 11045-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11045-2-AP (same clone as 11045-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11045-2-AP (same clone as 11045-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11045-2-AP (GNB5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

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Product Information

11045-2-PBS targets GNB5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNB5 fusion protein Ag1518
• Full Name: guanine nucleotide binding protein (G protein), beta 5
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC011671
• Gene Symbol: GNB5
• Gene ID (NCBI): 10681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.