GNB4 Polyclonal antibody

GNB4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11978-2-AP

Synonyms

HG2B, Guanine nucleotide-binding protein subunit beta-4, CMTD1F

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Validation Data Gallery

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  WB analysis using 11978-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11978-2-AP (GNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11978-2-AP

  IP result of anti-GNB4 (IP:11978-2-AP, 4ug; Detection:11978-2-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of SH-SY5Y using 11978-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 11978-2-AP (GNB4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, MCF-7 cells, SH-SY5Y cells, human placenta tissue, mouse kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11978-2-AP targets GNB4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNB4 fusion protein Ag2604
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 4
• Calculated Molecular Weight: 340 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000873
• Gene Symbol: GNB4
• Gene ID (NCBI): 59345
• RRID: AB_2109620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G protein subunit beta 4 (GNB4), is one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins. GNB4 is important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

Publications

Species	Application	Title
human	IF	BMC Med; Helicobacter pylori-induced aberrant demethylation and expression of GNB4 promotes gastric carcinogenesis via the Hippo-YAP1 pathway; Authors - Duanrui Liu
human	WB	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh