GNB3 Recombinant antibody, PBS Only

GNB3 Uni-rAb^TM Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240281D1

Cat no : 83452-4-PBS

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 240281D1

---

Validation Data Gallery

• 

  WB analysis using 83452-4-RR (same clone as 83452-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83452-4-RR (GNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83452-4-RR (same clone as 83452-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83452-4-RR (same clone as 83452-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83452-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83452-4-RR against Human GNB3 were performed. The affinity constant is below 1 pM.

---

Product Information

The immunogen of 83452-4-PBS is GNB3 Fusion Protein expressed in E. coli.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GNB3 fusion protein Ag0127
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC000115
• Gene Symbol: GNB3
• Gene ID (NCBI): 2784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.