GNB3 Polyclonal antibody
GNB3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15388-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, HepG2 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
15388-1-AP targets GNB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GNB3 fusion protein Ag7050 |
Full Name | guanine nucleotide binding protein (G protein), beta polypeptide 3 |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 35-37 kDa |
GenBank Accession Number | BC002454 |
Gene Symbol | GNB3 |
Gene ID (NCBI) | 2784 |
RRID | AB_2109603 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.
Protocols
Product Specific Protocols | |
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WB protocol for GNB3 antibody 15388-1-AP | Download protocol |
IHC protocol for GNB3 antibody 15388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |