GNB3 Polyclonal antibody

GNB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15388-1-AP

Synonyms

GNB3, Transducin beta chain 3

---

Validation Data Gallery

• 

  WB analysis using 15388-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15388-1-AP (GNB3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15388-1-AP targets GNB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNB3 fusion protein Ag7050
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC002454
• Gene Symbol: GNB3
• Gene ID (NCBI): 2784
• RRID: AB_2109603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.