GNAS Monoclonal antibody, PBS Only

GNAS Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9C10

Cat no : 66253-1-PBS

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH, Protein ALEX

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Validation Data Gallery

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  WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66253-1-Ig (same clone as 66253-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66253-1-PBS targets GNAS in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNAS fusion protein Ag20530
• Full Name: GNAS complex locus
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002722
• Gene Symbol: GNAS
• Gene ID (NCBI): 2778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.