GNAQ Polyclonal antibody

• Featured Product
• KD/KO Validated

GNAQ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 13927-1-AP

Synonyms

Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q, GAQ, G ALPHA q, CMC1

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Validation Data Gallery

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  WB analysis using 13927-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13927-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 13927-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13927-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13927-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HEK-293 using 13927-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse lung tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

13927-1-AP targets GNAQ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAQ fusion protein Ag4947
• Full Name: guanine nucleotide binding protein (G protein), q polypeptide
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC057777
• Gene Symbol: GNAQ
• Gene ID (NCBI): 2776
• RRID: AB_2111647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Publications

Species	Application	Title
mouse	WB	J Exp Med; Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.; Authors - Miwa Sasai
mouse	WB,IF	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
mouse	WB	Oncogene; Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.; Authors - Yongyun Li
human	WB	Proteomics; iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.; Authors - Hao Chen
human	WB,IHC	Oncol Rep; G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.; Authors - Yizhuo Wang ; KD Validated
human	WB,IF	J Biol Chem; Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890; Authors - Clinita E Randolph