Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11641-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11641-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11641-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 11641-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11641-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNAI3 antibody (11641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNAI3 antibody (11641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of A431 using 11641-1-AP
1x10^6 A431 cells were intracellularly stained with 0.4 ug GNAI3 Polyclonal antibody (11641-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 A431 cells were intracellularly stained with 0.4 ug GNAI3 Polyclonal antibody (11641-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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rat kidney tissue, A431 cells, mouse brain tissue, human kidney tissue
Positive IHC detected in
human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive FC (Intra) detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)
RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.
Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11641-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11641-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11641-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 11641-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11641-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11641-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11641-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).
IF/ICC Figures
IF Staining of HeLa using 11641-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNAI3 antibody (11641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC (INTRA) Figures
FC experiment of A431 using 11641-1-AP
1x10^6 A431 cells were intracellularly stained with 0.4 ug GNAI3 Polyclonal antibody (11641-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
GNAI3 Polyclonal antibody
Catalog Number
11641-1-AP
Citations
2
Dilutions
WB : 1:5000-1:50000 IHC : 1:20-1:200 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
