GNAI2 Polyclonal antibody

GNAI2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11136-1-AP

Synonyms

H_LUCA16.1, H_LUCA15.1, Guanine nucleotide-binding protein G(i) subunit alpha-2, GNAI2B, GIP

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Validation Data Gallery

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  WB analysis using 11136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 11136-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11136-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11136-1-AP

  IP result of anti-GNAI2 (IP:11136-1-AP, 4ug; Detection:11136-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse ovary using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11136-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:10 (under 25x lens).

• 

  IF Staining of HeLa using 11136-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI2 antibody (11136-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, SKOV-3 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, human kidney tissue, human lung cancer tissue, mouse ovary tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

11136-1-AP targets GNAI2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAI2 fusion protein Ag1597
• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC012138
• Gene Symbol: GNAI2
• Gene ID (NCBI): 2771
• RRID: AB_2232442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Publications

Species	Application	Title
mouse	WB	Nat Commun; EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.; Authors - Katie S Kindt
mouse	IF	Elife; Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis; Authors - Amandine Jarysta
human	WB	Oncotarget; PMID: 24658464; Authors - Jie Li
mouse	IF	bioRxiv; Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis; Authors - Amandine Jarysta
mouse	WB	Adv Sci (Weinh); Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC; Authors - Xinyuan Zhao
human	WB,CoIP	Sci Adv; RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells; Authors - Anil Akturk