GNA14 Polyclonal antibody

GNA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13350-1-AP

Synonyms

Guanine nucleotide-binding protein subunit alpha-14, G-protein subunit alpha-14, G alpha-14

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Validation Data Gallery

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  WB analysis of mouse lung using 13350-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13350-1-AP (GNA14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13350-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13350-1-AP (GNA14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13350-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13350-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13350-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13350-1-AP (GNA14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13350-1-AP

  Immunofluorescent analysis of HepG2 cells, using GNA14 antibody 13350-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13350-1-AP targets GNA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNA14 fusion protein Ag3930
• Full Name: guanine nucleotide binding protein (G protein), alpha 14
• Calculated Molecular Weight: 355 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC027886
• Gene Symbol: GNA14
• Gene ID (NCBI): 9630
• RRID: AB_2877942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.