GMPS Polyclonal antibody

GMPS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16376-1-AP

Synonyms

Glutamine amidotransferase, GMP synthetase, GMPS

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Validation Data Gallery

• 

  WB analysis of HeLa using 16376-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16376-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16376-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16376-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16376-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16376-1-AP

  IP result of anti-GMPS (IP:16376-1-AP, 4ug; Detection:16376-1-AP 1:800) with HeLa cells lysate 1080ug.

• 

  IHC staining of human liver cancer using 16376-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 16376-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 3 publications below

Product Information

16376-1-AP targets GMPS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMPS fusion protein Ag9350
• Full Name: guanine monphosphate synthetase
• Calculated Molecular Weight: 693 aa, 77 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC012178
• Gene Symbol: GMPS
• Gene ID (NCBI): 8833
• RRID: AB_2232412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB	Aging (Albany NY); The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.; Authors - Jing Wang
human	WB,IHC	J Biomed Sci; TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.; Authors - Panpan Zhang
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles
human,mouse	WB,IHC	J Proteome Res; Comprehensive Urinary Proteome Profiling Analysis Identifies Diagnosis and Relapse Surveillance Biomarkers for Bladder Cancer; Authors - Qi Chang