GMPR 1/2 Polyclonal antibody

GMPR 1/2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15683-1-AP

Synonyms

GMP reductase 1, GMPR, GMPR1, GMPR2

---

Validation Data Gallery

• 

  WB analysis of mouse skin using 15683-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15683-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15683-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 15683-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse skin tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15683-1-AP targets GMPR 1/2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMPR 1/2 fusion protein Ag8185
• Full Name: guanosine monophosphate reductase
• Calculated Molecular Weight: 345 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC008281
• Gene Symbol: GMPR
• Gene ID (NCBI): 2766
• RRID: AB_2111072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMPR (Guanosine monophosphate reductase) catalyzes the irreversible NADPH-dependent reductive deamination of guanosine monophosphate (GMP) to inosine monophosphate (IMP). GMPR is able to convert guanosine nucleotides to the pivotal precursor of both guanine (G) and adenine (A) nucleotides. It plays an important role in maintaining the intracellular balance of A and G nucleotides. This antibody can recognize both GMPR1 and GMPR2 due to the high homology.

Publications

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
human	WB,IF,IP	Cell Chem Biol; Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.; Authors - David W Wolff
mouse	WB	Cancer Prev Res (Phila); Reducing Fatty Acid Oxidation Improves Cancer-free Survival in a Mouse Model of Li-Fraumeni Syndrome.; Authors - Ping-Yuan Wang
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles