GMPPB Polyclonal antibody

• Featured Product
• KD/KO Validated

GMPPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15094-1-AP

Synonyms

Mannose-1-phosphate guanyltransferase beta, KIAA1851, GTP-mannose-1-phosphate guanylyltransferase beta, GDP-mannose pyrophosphorylase B, EC:2.7.7.13

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 15094-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15094-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 15094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15094-1-AP (GMPPB antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 15094-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15094-1-AP (GMPPB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

15094-1-AP targets GMPPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMPPB fusion protein Ag7168
• Full Name: GDP-mannose pyrophosphorylase B
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC001141
• Gene Symbol: GMPPB
• Gene ID (NCBI): 29925
• RRID: AB_2111064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMPPB is a cytoplasmic enzyme that catalyzes the formation of GDP-mannose, a key substrate for several glycosylation pathways, including O-mannosylation of α-DG. GMPPB facilitates the formation of the sugar donor GDP-mannose and pyrophosphate from mannose-1-phosphate and GTP, a very early step in the glycosylation pathway (PMID: 37834154, 27147698). GMPPB mutations correlate with several congenital diseases, including severe congenital muscular dystrophy (CMD) with abnormalities in the brain and eye (PMID: 26310427, 30684953). Western blot analysis detected GMPPB at an apparent molecular mass of 40 kDa.

Publications

Species	Application	Title
human	WB	iScience; A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.; Authors - Norio Sasaoka
mouse	WB	Front Mol Biosci; Altered Glycosylation in the Aging Heart.; Authors - Patricia Franzka
zebrafish	WB	Mol Biomed; GMPPB-congenital disorders of glycosylation associate with decreased enzymatic activity of GMPPB.; Authors - Zhe Liu
human,mouse	WB	Front Nutr; Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12; Authors - Patricia Franzka
human	WB,IHC	Int J Mol Sci; Silencing GMPPB Inhibits the Proliferation and Invasion of GBM via Hippo/MMP3 Pathways; Authors - Zi-Lu Huang ; KD Validated
mouse	WB,IF	Front Mol Neurosci; Consequences of GMPPB deficiency for neuromuscular development and maintenance; Authors - Mona K Schurig