GMF Beta Polyclonal antibody

• Featured Product
• KD/KO Validated

GMF Beta Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10690-1-AP

Synonyms

GMFB, GMF-β, GMF-beta, GMF, glia maturation factor, beta

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Validation Data Gallery

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  WB analysis using 10690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF Beta antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10690-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10690-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:30 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10690-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10690-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10690-1-AP (GMF-beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10690-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10690-1-AP (GMF-beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10690-1-AP (GMF-beta antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of U-87 MG using 10690-1-AP

  1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug Anti-Human GMF Beta (10690-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, human brain tissue, PC-12 cells, mouse heart tissue, rat brain tissue, SH-SY5Y cells, U-87 MG cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

10690-1-AP targets GMF Beta in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMF Beta fusion protein Ag1104
• Full Name: glia maturation factor, beta
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC005359
• Gene Symbol: GMFB
• Gene ID (NCBI): 2764
• RRID: AB_2110837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon. GMFB was positive in the cytoplasm and nuclei of all cells in whole brain.

Publications

Species	Application	Title
human	WB	Int J Biol Macromol; SUMOylation of GMFB regulates its stability and function in retinal pigment epithelial cells under hyperglycemia; Authors - Wan Sun
mouse	IHC	Development; Coronary veins determine the pattern of sympathetic innervation in the developing heart.; Authors - Nam Joseph J
human	IHC	Int J Mol Sci; Parkinson's Disease Dementia Patients: Expression of Glia Maturation Factor in the Brain; Authors - Ramasamy Thangavel
human	WB,IHC	Eur J Cancer; Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.; Authors - Li Yan Li YL
human	WB	Front Oncol; Glia Maturation Factor Beta as a Novel Biomarker and Therapeutic Target for Hepatocellular Carcinoma.; Authors - Wan Sun
human	WB,IF	Front Oncol; Glia Maturation Factor β as a Novel Independent Prognostic Biomarker and Potential Therapeutic Target of Kidney Renal Clear Cell Carcinoma.; Authors - Tong Zhu