GMF Beta Monoclonal antibody, PBS Only

GMF Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B10A2

Cat no : 60062-1-PBS

Synonyms

glia maturation factor beta, GMF Beta, GMFB, GMF-beta

---

Validation Data Gallery

• 

  WB analysis of fetal human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 60062-1-Ig (same clone as 60062-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-87 MG using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using GMF Beta antibody (60062-1-Ig, Clone: 3B10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60062-1-Ig (same clone as 60062-1-PBS)

  Immunofluorescent analysis of Hela cells, using GMFB antibody 60062-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

---

Product Information

60062-1-PBS targets GMF Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GMF Beta fusion protein Ag1104
• Full Name: glia maturation factor, beta
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC005359
• Gene Symbol: GMFB
• Gene ID (NCBI): 2764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.