GOLGA2/GM130 Recombinant antibody

• Featured Product
• KD/KO Validated

GOLGA2/GM130 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1M17

Cat no : 82705-8-RR

Synonyms

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 1M17

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Validation Data Gallery

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  WB analysis using 82705-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82705-8-RR

  WB result of GOLGA2/GM130 antibody (82705-8-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.

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  IHC staining of rat kidney using 82705-8-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 82705-8-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82705-8-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82705-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82705-8-RR against Human GM130/GOLGA2 were performed. The affinity constant is 0.942 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8400
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82705-8-RR targets GOLGA2/GM130 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GM130
• Gene ID (NCBI): 2801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)