GLUT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLUT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 27571-1-AP

Synonyms

Fructose transporter, GLUT 5, GLUT5, SLC2A5

---

Validation Data Gallery

• 

  WB analysis of A549 using 27571-1-AP

  WB result of GLUT5 antibody (27571-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLUT5 transfected A549 cells.

• 

  WB analysis of Caco-2 using 27571-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 27571-1-AP (GLUT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27571-1-AP (GLUT5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27571-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27571-1-AP (GLUT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27571-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27571-1-AP (GLUT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27571-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27571-1-AP (GLUT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27571-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27571-1-AP (GLUT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Caco-2 cells, mouse brain tissue, A549 cells, mouse kidney tissue, rat testis tissue
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 3 publications below

Product Information

27571-1-AP targets GLUT5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLUT5 fusion protein Ag25682
• Full Name: solute carrier family 2 (facilitated glucose/fructose transporter), member 5
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 48-70 kDa
• GenBank Accession Number: BC001820
• Gene Symbol: GLUT5
• Gene ID (NCBI): 6518
• RRID: AB_2880913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUT5, also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen (PMID: 12750891). GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue (adipocytes), and brain (PMID: 9781312, 18398011).

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Nutrients; Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice.; Authors - Kai Aoki
mouse	WB	iScience; Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.; Authors - Hu Hua
human	WB	Dev Neurobiol; Prestin-prestin and prestin-GLUT5 interactions in HEK293T cells.; Authors - Xudong Wu
mouse	WB	Cell Metab; Dietary fructose-mediated adipocyte metabolism drives antitumor CD8+ T cell responses; Authors - Yuerong Zhang
mouse,human	WB	Anim Cells Syst (Seoul); Role of Carbohydrate response element-binding protein in mediating dexamethasone-induced glucose transporter 5 expression in Caco-2BBE cells and during the developmental phase in mice; Authors - Soonjae Hwang