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GLUT5 Polyclonal antibody

GLUT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27571-1-AP

Synonyms

Fructose transporter, GLUT 5, GLUT5, SLC2A5



Tested Applications

Positive WB detected inCaco-2 cells, mouse brain tissue, A549 cells, mouse kidney tissue, rat testis tissue
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27571-1-AP targets GLUT5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLUT5 fusion protein Ag25682
Full Name solute carrier family 2 (facilitated glucose/fructose transporter), member 5
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 48-70 kDa
GenBank Accession NumberBC001820
Gene Symbol GLUT5
Gene ID (NCBI) 6518
RRIDAB_2880913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUT5, also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen (PMID: 12750891). GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue (adipocytes), and brain (PMID: 9781312, 18398011).

Protocols

Product Specific Protocols
WB protocol for GLUT5 antibody 27571-1-APDownload protocol
IHC protocol for GLUT5 antibody 27571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Cell Metab

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Authors - Peng Zhou
mouseWB

Nutrients

Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice.

Authors - Kai Aoki
mouseWB

iScience

Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.

Authors - Hu Hua
humanWB

Dev Neurobiol

Prestin-prestin and prestin-GLUT5 interactions in HEK293T cells.

Authors - Xudong Wu
mouseWB

Cell Metab

Dietary fructose-mediated adipocyte metabolism drives antitumor CD8+ T cell responses

Authors - Yuerong Zhang
mouse,humanWB

Anim Cells Syst (Seoul)

Role of Carbohydrate response element-binding protein in mediating dexamethasone-induced glucose transporter 5 expression in Caco-2BBE cells and during the developmental phase in mice

Authors - Soonjae Hwang