GLUT4 Monoclonal antibody, PBS Only

GLUT4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G7C9

Cat no : 66846-1-PBS

Synonyms

SLC2A4, GLUT-4, GLUT 4, Glucose transporter type 4, insulin-responsive, Glucose transporter GLUT 4

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Validation Data Gallery

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  WB analysis using 66846-1-Ig (same clone as 66846-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  WB analysis using 66846-1-Ig (same clone as 66846-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66846-1-Ig (same clone as 66846-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 66846-1-Ig (same clone as 66846-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 66846-1-Ig (same clone as 66846-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 66846-1-Ig (same clone as 66846-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GLUT4 (66846-1-Ig, Clone:3G7C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66846-1-PBS in a different storage buffer formulation.

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Product Information

66846-1-PBS targets GLUT4 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, mouse, human samples.

• Tested Reactivity: pig, rat, mouse, human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLUT4 fusion protein Ag15390
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 4
• Calculated Molecular Weight: 509 aa, 55 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC069615
• Gene Symbol: GLUT4
• Gene ID (NCBI): 6517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glucose transporter 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4), is a transporter protein regulating glucose transport across cell membranes in an INS-dependent manner.

What is the molecular weight of GLUT4? Is GLUT4 post-translationally modified?

The molecular weight of GLUT4 transporter is 55 kDa. GLUT4 can be N-glycosylated, which is important for its stability and trafficking between the recycling compartment and plasma membrane (PMID: 21757715 and 22545627), and it can also be ubiquitinated and phosphorylated (PMID: 23665900).

What is the subcellular localization of GLUT4?

Glucose transporters, including GLUT4, are multiple-pass integral membrane proteins. GLUT4 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes. The localization of GLUT4 depends on stimulation with  INS - in basal conditions GLUT4 is retained intracellularly, while upon  INS stimulation it is translocated to the plasma membrane (PMID: 18570632).

What molecules can be transported by GLUT4?

Although the main substrate of GLUT4 transport is glucose, it can also transport glucosamine.

What is the tissue expression pattern of GLUT4?

GLUT4 is expressed in white and brown adipose tissue and in muscle and heart cells, where it is the main glucose transporter responsible for peripheral glucose uptake in response to INS.

Which cell lines can be used to study insulin-dependent GLUT4 protein translocation in glucose uptake assays?

Fat and muscle cells are primarily used in glucose uptake assays because they physiologically respond to  INS(PMID: 26646194). The most commonly used cell lines are 3T3-L1 cells (murine pre-adipose fibroblasts), L6 cells (rat myoblasts), and C2C12 cells (murine myoblasts).