GLUT2 Monoclonal antibody, PBS Only

GLUT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8B1

Cat no : 66889-1-PBS

Synonyms

GLUT 2, GLUT2, SLC2A2

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Validation Data Gallery

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  WB analysis of 4T1 using 66889-1-Ig (same clone as 66889-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66889-1-Ig (same clone as 66889-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66889-1-Ig (same clone as 66889-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66889-1-Ig (same clone as 66889-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66889-1-Ig (same clone as 66889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66889-1-Ig (same clone as 66889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66889-1-PBS targets GLUT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLUT2 fusion protein Ag14551
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC060041
• Gene Symbol: SLC2A2
• Gene ID (NCBI): 6514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GLUT2 is a member of glucose transporters or GLUTs which catalyze the glucose transport across plasma membrane. GLUT2 is selectively expressed in hepatocytes, pancreatic beta cells, and absorptive renal and intestinal epithelial cells. GLUT2 is required to maintain normal glucose homeostasis and normal function and development of the endocrine pancreas. Its absence leads to symptoms characteristic of non-insulin-dependent diabetes mellitus.