GLUD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLUD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14462-1-AP

Synonyms

GLUDP1, GLUD 2, GDH2, GDH 2, EC:1.4.1.3

---

Validation Data Gallery

• 

  WB analysis using 14462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14462-1-AP

  WB result of GLUD2 antibody (14462-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLUD2 transfected HepG2 cells.

• 

  IHC staining of human prostate cancer using 14462-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 14462-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14462-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GLUD2 antibody (14462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, HepG2 cells，U-251 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14462-1-AP targets GLUD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLUD2 fusion protein Ag5748
• Full Name: glutamate dehydrogenase 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC050732
• Gene Symbol: GLUD2
• Gene ID (NCBI): 2747
• RRID: AB_2110642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human	IHC	Transl Oncol; Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.; Authors - Xuyong Wei
human	WB	Amino Acids; PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.; Authors - Chunhong Cui