GLUD1 Monoclonal antibody, PBS Only

GLUD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G10D3

Cat no : 67026-1-PBS

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

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Validation Data Gallery

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  WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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Product Information

67026-1-PBS targets GLUD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLUD1 fusion protein Ag6179
• Full Name: glutamate dehydrogenase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC040132
• Gene Symbol: GLUD1
• Gene ID (NCBI): 2746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.