GLTSCR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLTSCR2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 27353-1-AP

Synonyms

GLT, GLTSCR2, NOP53, p60, PICT 1, PICT1

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Validation Data Gallery

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  WB analysis using 27353-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27353-1-AP (GLTSCR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 27353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GLTSCR2 antibody (27353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse pancreas tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

27353-1-AP targets GLTSCR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLTSCR2 fusion protein Ag26358
• Full Name: glioma tumor suppressor candidate region gene 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC010095
• Gene Symbol: GLTSCR2
• Gene ID (NCBI): 29997
• RRID: AB_2880852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing; Authors - Sotaro Miyao ; KD Validated
human		bioRxiv; Nucleolar dynamics are determined by the ordered assembly of the ribosome; Authors - Jessica Sheu-Gruttadauria
human	WB	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (03-15-2022)

U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: U2OS (Human Osteosarcoma) cells

GLTSCR2 Antibody Immunofluorescence validation (1:200 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:27353-1-AP)