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  • KD/KO Validated

GLTSCR2 Polyclonal antibody

GLTSCR2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27353-1-AP

Synonyms

GLT, GLTSCR2, NOP53, p60, PICT 1, PICT1



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27353-1-AP is GLTSCR2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLTSCR2 fusion protein Ag26358
Full Name glioma tumor suppressor candidate region gene 2
Calculated molecular weight 54 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC010095
Gene symbol GLTSCR2
Gene ID (NCBI) 29997
RRIDAB_2880852
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLTSCR2 antibody 27353-1-APDownload protocol
IHC protocol for GLTSCR2 antibody 27353-1-APDownload protocol
IF protocol for GLTSCR2 antibody 27353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing

Authors - Sotaro Miyao
  • KD Validated
human

bioRxiv

Nucleolar dynamics are determined by the ordered assembly of the ribosome

Authors - Jessica Sheu-Gruttadauria
humanWB

Dev Cell

Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method

Authors - Sarah Carden

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elisa (Verified Customer) (03-15-2022)

U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: U2OS (Human Osteosarcoma) cells
GLTSCR2 Antibody Immunofluorescence validation (1:200 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:27353-1-AP)