Validation Data Gallery
|Positive WB detected in
|human placenta tissue
|Positive IHC detected in
|human colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 27353-1-AP is GLTSCR2 Fusion Protein expressed in E. coli.
|Host / Isotype
|Rabbit / IgG
|GLTSCR2 fusion protein Ag26358
|glioma tumor suppressor candidate region gene 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Biochem Biophys Res Commun
MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing
Nucleolar dynamics are determined by the ordered assembly of the ribosome
Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method
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Elisa (Verified Customer) (03-15-2022)
U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).