GLS2 Polyclonal antibody

GLS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20171-1-AP

Synonyms

GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA

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Validation Data Gallery

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  WB analysis using 20171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20171-1-AP (GLS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20171-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20171-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20171-1-AP targets GLS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLS2 fusion protein Ag14003
• Full Name: glutaminase 2 (liver, mitochondrial)
• Calculated Molecular Weight: 602 aa, 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC059370
• Gene Symbol: GLS2
• Gene ID (NCBI): 27165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS2, also named as GA and GLS, belongs to the glutaminase family. It is Glutaminase liver isoform . GLS2 plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. GLS2 may play a role in preventing tumor proliferation. This antibody is specific to GLS2.