WB result of GLS antibody (81486-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLS transfected A549 cells.
WB analysis using 81486-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 81486-1-RR (GLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 81486-1-RR (GLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 81486-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81486-1-RR (GLS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
rat brain tissue, mouse brain tissue
Positive IF/ICC detected in
A549 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
IRF5 mediates adaptive immunity via altered glutamine metabolism, mTORC1 signaling and post-transcriptional regulation following T cell receptor activation
Hypoxia-inducible factor-2α promotes fibrosis in non-alcoholic fatty liver disease by enhancing glutamine catabolism and inhibiting yes-associated protein phosphorylation in hepatic stellate cells
WB result of GLS antibody (81486-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLS transfected A549 cells.
WB analysis using 81486-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 81486-1-RR (GLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 81486-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81486-1-RR (GLS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse brain using 81486-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KGA/GAC antibody (81486-1-RR, Clone: 4C20 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat brain using 81486-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using KGA/GAC antibody (81486-1-RR, Clone: 4C20 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A549 using 81486-1-RR
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using GLS antibody (81486-1-RR, Clone: 4C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
AFFINITY Figures
Affinity and Kinetic Characterization of 81486-1-RR
Biolayer interferometry (BLl) kinetic assays of 81486-1-RR against Human GLS were performed. The affinity constant is 0.182 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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