GLP1R Polyclonal antibody

• Featured Product
• KD/KO Validated

GLP1R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 26196-1-AP

Synonyms

GLP-1-R, GLP-1R, GLP-1 receptor, GLP 1R, GLP 1 receptor

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Validation Data Gallery

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  WB analysis using 26196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26196-1-AP (GLP1R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 26196-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26196-1-AP (GLP1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

26196-1-AP targets GLP1R in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLP1R fusion protein Ag23529
• Full Name: glucagon-like peptide 1 receptor
• Calculated Molecular Weight: 463 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC112126
• Gene Symbol: GLP1R
• Gene ID (NCBI): 2740
• RRID: AB_2880421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLP1R (glucagon-like peptide-1 receptor) is a G protein-coupled receptor belonging to the secretin receptor super-family, also known as class B (PMID:34911028). GLP1R is widely distributed in pancreatic islets, muscles, gastrointestinal tract, lung, liver, pancreas, and other tissues or organs (PMID:35989517). Moreover, GLP1R agonist has been reported to induce autophagy of EC (Endometrial carcinoma) cells, and high GLP1R expression may be associated with good prognosis of EC patients, suggesting that GLP1R is likely to participate in the progression of EC (PMID:29907137).

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Geniposide Improves Diabetic Nephropathy by Enhancing ULK1-Mediated Autophagy and Reducing Oxidative Stress through AMPK Activation.; Authors - Theodomir Dusabimana
rat	WB	Life Sci; Human gestational diabetes mellitus-derived exosomes impair glucose homeostasis in pregnant mice and stimulate functional maturation of offspring-islets; Authors - Linhai Zou
mouse	WB	Biomed Pharmacother; Liraglutide, a glucagon-like peptide-1 receptor agonist, suppresses osteoclastogenesis through the inhibition of NF-κB and MAPK pathways via GLP-1R.; Authors - Ziyi Li ; KD Validated
	WB	Front Pharmacol; Novel Small Molecule Glucagon-Like Peptide-1 Receptor Agonist S6 Stimulates Insulin Secretion From Rat Islets.; Authors - Xiaohua Yang
rat	WB	Front Pharmacol; A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.; Authors - Ting Cao
rat	WB	Front Endocrinol (Lausanne); Clozapine Induced Disturbances in Hepatic Glucose Metabolism: The Potential Role of PGRMC1 Signaling.; Authors - Ting Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (08-16-2023)

This KO validated GLP-1R antibody generated specific robust signals in mouse pancreas tissue when used for immunofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other