GLOD4 Polyclonal antibody

GLOD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16788-1-AP

Synonyms

Glyoxalase domain-containing protein 4, GLOD 4, CGI-150, CGI150, CGI 150

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Validation Data Gallery

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  WB analysis using 16788-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16788-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16788-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 16788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16788-1-AP (GLOD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16788-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16788-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse ovary using 16788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16788-1-AP (GLOD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 16788-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16788-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, human placenta tissue, rat brain tissue
• Positive IHC detected in: human heart tissue, mouse ovary tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

16788-1-AP targets GLOD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLOD4 fusion protein Ag10222
• Full Name: glyoxalase domain containing 4
• Calculated Molecular Weight: 289 aa, 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008605
• Gene Symbol: GLOD4
• Gene ID (NCBI): 51031
• RRID: AB_2247517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
monkey	IHC	Mol Biosyst; Unravelling the proteome of adult rhesus monkey ovaries.; Authors - Hui He