GLIS3 Polyclonal antibody, PBS Only

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  WB analysis of mouse kidney using 12678-1-AP (same clone as 12678-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12678-1-AP (same clone as 12678-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 12678-1-AP (same clone as 12678-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12678-1-AP (same clone as 12678-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

GLIS3 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 12678-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

12678-1-PBS targets GLIS3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLIS3 fusion protein Ag3366
• Full Name: GLIS family zinc finger 3
• Calculated Molecular Weight: 775 aa, 84 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC033899
• Gene Symbol: GLIS3
• Gene ID (NCBI): 169792
• RRID: AB_2877873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEA6
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. GLIS3 (gLIS family zinc finger 3), also known as ZNF515 that localizes to the nucleus and contains five C2H2-type zinc fingers. Expressed in a variety of tissues, including kidney, brain, liver, lung, ovary, pancreas, thymus and skeletal muscle, GLIS3 functions as both an activator and a suppressor of transcription, specifically binding the consensus sequence 5'-GACCACCCAC-3' through its C2H2-type zinc fingers. Defects in the gene encoding GLIS3 are a cause of NDH syndrome; a neonatal diabetes that is characterized by congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. There are various isoform of GLIS3 and molecular weight of one isoform is 70 kDa.