GLIS1 Polyclonal antibody

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  WB analysis of Jurkat using 23138-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23138-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 23138-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 23138-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GLIS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23138-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GLI similar 1, GLIS family zinc finger 1, GLIS1, Zinc finger protein GLIS1

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

23138-1-AP targets GLIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLIS1 fusion protein Ag19214
• Full Name: GLIS family zinc finger 1
• Calculated Molecular Weight: 620 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC101799
• Gene Symbol: GLIS1
• Gene ID (NCBI): 148979
• RRID: AB_2879216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8NBF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Reprod Sci; Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis; Authors - Jiazhen Huang
human	WB,IHC	BMC Biol; N6-methyladenosine regulates metabolic remodeling in kidney aging through transcriptional regulator GLIS1; Authors - Li Xu