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GLI4 Polyclonal antibody, PBS Only

GLI4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16551-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GLI4 Polyclonal antibody (16551-1-AP)

    WB analysis of Jurkat using 16551-1-AP (same clone as 16551-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16551-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLI4 Polyclonal antibody (16551-1-AP)

    WB analysis using 16551-1-AP (same clone as 16551-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16551-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using GLI4 Polyclonal antibody (16551-1-AP)

    IF Staining of A549 using 16551-1-AP (same clone as 16551-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using GLI4 antibody (16551-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16551-1-PBS in a different storage buffer formulation.

Product Information

16551-1-PBS targets GLI4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLI4 fusion protein Ag9497
Full Name GLI family zinc finger 4
Calculated Molecular Weight 376 aa, 41 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC014165
Gene Symbol GLI4
Gene ID (NCBI) 2738
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.