Alpha Galactosidase A Monoclonal antibody

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• KD/KO Validated

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2B2C5

Cat no : 66121-1-Ig

Synonyms

GLA, GALA, EC:3.2.1.22, Alpha-galactosidase A, Alpha-D-galactoside galactohydrolase

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Validation Data Gallery

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  WB analysis of HEK-293 using 66121-1-Ig

  WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells..

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  WB analysis using 66121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of LNCaP using 66121-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66121-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66121-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66121-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 66121-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66121-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HepG2 using 66121-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha Galactosidase A antibody (66121-1-Ig, Clone: 2B2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, human kidney tissue, LNCaP cells, A431 cells
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66121-1-Ig targets Alpha Galactosidase A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Galactosidase A fusion protein Ag7505
• Full Name: galactosidase, alpha
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC002689
• Gene Symbol: GLA
• Gene ID (NCBI): 2717
• RRID: AB_2881520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Publications

Species	Application	Title
	WB	bioRxiv; Antigen-display exosomes provide adjuvant-free protection against SARS-CoV-2 disease at nanogram levels of spike protein; Authors - Chenxu Guo
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland