Alpha Galactosidase A Polyclonal antibody

• Featured Product
• KD/KO Validated

Alpha Galactosidase A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15428-1-AP

Synonyms

Alpha D galactosidase A, Alpha galactosidase A, galactosidase, alpha

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 15428-1-AP

  WB result of GLA antibody (15428-1-AP, 1:600) with si-Control and si-GLA transfected HEK293 cells..

• 

  WB analysis using 15428-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15428-1-AP

  IP result of anti-Alpha Galactosidase A (IP:15428-1-AP, 3ug; Detection:15428-1-AP 1:1000) with HEK-293 cells lysate 1800ug.

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15428-1-AP (Alpha galactosidase A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15428-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15428-1-AP (Alpha galactosidase A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15428-1-AP targets Alpha Galactosidase A in WB, IP, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Galactosidase A fusion protein Ag7609
• Full Name: galactosidase, alpha
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC002689
• Gene Symbol: GLA
• Gene ID (NCBI): 2717
• RRID: AB_10638002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLA (Alpha-galactosidase A), also named as Melibiase or Agalsidase, belongs to the glycosyl hydrolase 27 family. It catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. The deficient activity of GLA can cause Fabry disease which is an X-linked inborn error of glycosphingolipid metabolis (PMID: 19287194). Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease (PMID: 20398385). In humans, GLA is synthesized as a 50 kDa precursor, which is further processed to a 46 kDa mature form of the protein (PMID: 9883849, 19387866). It also has a homodimer form with the molecular mass of 110 kDa (PMID: 17287429).

Publications

Species	Application	Title
human,mouse	WB,IHC	J Extracell Vesicles; Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders.; Authors - Joaquin Seras-Franzoso ; KO Validated
human		Orphanet J Rare Dis; Deep characterization of the anti-drug antibodies developed in Fabry disease patients, a prospective analysis from the French multicenter cohort FFABRY.; Authors - Wladimir Mauhin
rat	IF	Adv Mater; Sequential Targeted Enzyme-Instructed Self-Assembly Supramolecular Nanofibers to Attenuate Intervertebral Disc Degeneration; Authors - Yang Liu

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