Gastrokine 1 Polyclonal antibody

Gastrokine 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19344-1-AP

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

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Validation Data Gallery

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  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 19344-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 19344-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 19344-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human stomach tissue, mouse stomach tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Product Information

The immunogen of 19344-1-AP is Gastrokine 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gastrokine 1 fusion protein Ag6091
• Full Name: gastrokine 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC059778
• Gene Symbol: Gastrokine 1
• Gene ID (NCBI): 56287
• RRID: AB_10837226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Gastrokine-1, an anti-amyloidogenic protein secreted by the stomach, regulates diet-induced obesity.; Authors - Anne-Marie C Overstreet
human	WB,IHC	Helicobacter; Helicobacter pylori inhibits GKN1 expression via the CagA/p-ERK/AUF1 pathway.; Authors - Yanlei Guo
mouse	WB,IF,ELISA	Int J Biol Sci; IL-33 Accelerates Chronic Atrophic Gastritis through AMPK-ULK1 Axis Mediated Autolysosomal Degradation of GKN1; Authors - Kewei Liu
mouse	WB	Cell Mol Gastroenterol Hepatol; Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia; Authors - Pawan Puri