Gastrokine 1 Polyclonal antibody

Gastrokine 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14494-1-AP

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

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Validation Data Gallery

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  WB analysis of human stomach using 14494-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14494-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14494-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach cancer using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach cancer using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 14494-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 14494-1-AP (Gastrokine 1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human stomach tissue, mouse stomach tissue, rat stomach tissue
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14494-1-AP targets Gastrokine 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gastrokine 1 fusion protein Ag5898
• Full Name: gastrokine 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC059778
• Gene Symbol: Gastrokine 1
• Gene ID (NCBI): 56287
• RRID: AB_10863653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1 may play an important role in normal gastric function and may be a gastric tumour suppressor.