GJC2 Polyclonal antibody

GJC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55376-1-AP

Synonyms

Connexin 46.6, Connexin 47, CX46.6, Cx47, Gap junction alpha 12 protein, Gap junction gamma 2 protein, GJA12, GJC2, HLD2, PMLDAR, SPG44

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Validation Data Gallery

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  WB analysis of mouse brain using 55376-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55376-1-AP (GJC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 55376-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 55376-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 55376-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 55376-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55376-1-AP targets GJC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: gap junction protein, gamma 2, 47kDa
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_020435
• Gene Symbol: GJC2
• Gene ID (NCBI): 57165
• RRID: AB_2881317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.