GJB3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GJB3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12880-1-AP

Synonyms

EKV, DFNA2B, DFNA2, CX31, Connexin-31

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Validation Data Gallery

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  WB analysis of HeLa using 12880-1-AP

  WB result of GJB3 antibody (12880-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GJB3 transfected HeLa cells.

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  WB analysis using 12880-1-AP

  mouse stomach tissue was subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 12880-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12880-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12880-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12880-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12880-1-AP

  IP result of anti-GJB3 (IP:12880-1-AP, 4ug; Detection:12880-1-AP 1:500) with HeLa cells lysate 2600 ug.

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  IHC staining of human cervix using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse placenta using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12880-1-AP (GJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12880-1-AP (GJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12880-1-AP (GJB3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of human placenta using 12880-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using GJB3 antibody (12880-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse stomach tissue, mouse ovary tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse placenta tissue, human ovary tumor tissue, human cervix tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

12880-1-AP targets GJB3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, marmoset
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GJB3 fusion protein Ag3839
• Full Name: gap junction protein, beta 3, 31kDa
• Calculated Molecular Weight: 270 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC012918
• Gene Symbol: GJB3
• Gene ID (NCBI): 2707
• RRID: AB_2110907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).

Publications

Species	Application	Title
human	WB	Cell Rep; Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.; Authors - Makoto Hosoya
mouse	IHC	Stem Cells; FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development.; Authors - Masaaki Nishiyama
mouse	IF	J Neurosci; Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.; Authors - Teng-Wei Huang
marmoset	IF	Sci Rep; Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.; Authors - Makoto Hosoya
mouse	IF	Placenta; In vitro fertilization causes excessive glycogen accumulation in mouse placenta.; Authors - Jie Dong
mouse	IHC	J Pain Res; PMID: 29692624; Authors - Brice Bellessort

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